vector nti (version 7.1) software package Search Results


sc 71  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank sc 71
Sc 71, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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raw264 7 murine macrophages  (ATCC)
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ATCC raw264 7 murine macrophages
Raw264 7 Murine Macrophages, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli jm109 competent cells  (TaKaRa)
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TaKaRa escherichia coli jm109 competent cells
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pgl4.31[ luc2p/gal4uas/hygro ] vector  (Promega)
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Promega pgl4.31[ luc2p/gal4uas/hygro ] vector
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alexander stark  (Addgene inc)
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Addgene inc alexander stark
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pgem-t-easy vector  (Promega)
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Promega pgem-t-easy vector
Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescein labelled peanut agglutinin pna  (Vector Laboratories)
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Vector Laboratories fluorescein labelled peanut agglutinin pna
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irf1  (Vector Laboratories)
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Vector Laboratories irf1
Figure 6. SET7/9 Is Inhibited by Phosphorylation of the Target Peptide or Protein (A) Relative activity of SET7/9 on CelluSpot array derived from H3 peptides containing different combinations of PTMs (Table S2). Phosphorylation of T3, S10, and T11 abolished SET7/9 activity. (B) Influence of phosphorylation of nonhistone substrates on SET7/9 methyla- tion activity. GST-tagged nonhistone substrates of SET7/9 were incubated with PKA. All domains except Cullin1 were phosphorylated (Figure S5). Then, phosphorylated and mock treated domains were methylated by SET7/9. A complete loss or strong reduction of SET7/9 activity was observed with ZDN8, <t>IRF1,</t> MINT, PPARBP, and MeCP2. See also Figure S6.
Irf1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain elite 71 abc hrp kit  (Vector Laboratories)
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Vector Laboratories vectastain elite 71 abc hrp kit
Figure 6. SET7/9 Is Inhibited by Phosphorylation of the Target Peptide or Protein (A) Relative activity of SET7/9 on CelluSpot array derived from H3 peptides containing different combinations of PTMs (Table S2). Phosphorylation of T3, S10, and T11 abolished SET7/9 activity. (B) Influence of phosphorylation of nonhistone substrates on SET7/9 methyla- tion activity. GST-tagged nonhistone substrates of SET7/9 were incubated with PKA. All domains except Cullin1 were phosphorylated (Figure S5). Then, phosphorylated and mock treated domains were methylated by SET7/9. A complete loss or strong reduction of SET7/9 activity was observed with ZDN8, <t>IRF1,</t> MINT, PPARBP, and MeCP2. See also Figure S6.
Vectastain Elite 71 Abc Hrp Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcd5 expression vector  (Addgene inc)
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Addgene inc pcd5 expression vector
Figure 6. SET7/9 Is Inhibited by Phosphorylation of the Target Peptide or Protein (A) Relative activity of SET7/9 on CelluSpot array derived from H3 peptides containing different combinations of PTMs (Table S2). Phosphorylation of T3, S10, and T11 abolished SET7/9 activity. (B) Influence of phosphorylation of nonhistone substrates on SET7/9 methyla- tion activity. GST-tagged nonhistone substrates of SET7/9 were incubated with PKA. All domains except Cullin1 were phosphorylated (Figure S5). Then, phosphorylated and mock treated domains were methylated by SET7/9. A complete loss or strong reduction of SET7/9 activity was observed with ZDN8, <t>IRF1,</t> MINT, PPARBP, and MeCP2. See also Figure S6.
Pcd5 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector pcmv 7 1  (Addgene inc)
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Addgene inc vector pcmv 7 1
Figure 6. SET7/9 Is Inhibited by Phosphorylation of the Target Peptide or Protein (A) Relative activity of SET7/9 on CelluSpot array derived from H3 peptides containing different combinations of PTMs (Table S2). Phosphorylation of T3, S10, and T11 abolished SET7/9 activity. (B) Influence of phosphorylation of nonhistone substrates on SET7/9 methyla- tion activity. GST-tagged nonhistone substrates of SET7/9 were incubated with PKA. All domains except Cullin1 were phosphorylated (Figure S5). Then, phosphorylated and mock treated domains were methylated by SET7/9. A complete loss or strong reduction of SET7/9 activity was observed with ZDN8, <t>IRF1,</t> MINT, PPARBP, and MeCP2. See also Figure S6.
Vector Pcmv 7 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain elite abc kit  (Vector Laboratories)
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Vector Laboratories vectastain elite abc kit
Figure 6. SET7/9 Is Inhibited by Phosphorylation of the Target Peptide or Protein (A) Relative activity of SET7/9 on CelluSpot array derived from H3 peptides containing different combinations of PTMs (Table S2). Phosphorylation of T3, S10, and T11 abolished SET7/9 activity. (B) Influence of phosphorylation of nonhistone substrates on SET7/9 methyla- tion activity. GST-tagged nonhistone substrates of SET7/9 were incubated with PKA. All domains except Cullin1 were phosphorylated (Figure S5). Then, phosphorylated and mock treated domains were methylated by SET7/9. A complete loss or strong reduction of SET7/9 activity was observed with ZDN8, <t>IRF1,</t> MINT, PPARBP, and MeCP2. See also Figure S6.
Vectastain Elite Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 6. SET7/9 Is Inhibited by Phosphorylation of the Target Peptide or Protein (A) Relative activity of SET7/9 on CelluSpot array derived from H3 peptides containing different combinations of PTMs (Table S2). Phosphorylation of T3, S10, and T11 abolished SET7/9 activity. (B) Influence of phosphorylation of nonhistone substrates on SET7/9 methyla- tion activity. GST-tagged nonhistone substrates of SET7/9 were incubated with PKA. All domains except Cullin1 were phosphorylated (Figure S5). Then, phosphorylated and mock treated domains were methylated by SET7/9. A complete loss or strong reduction of SET7/9 activity was observed with ZDN8, IRF1, MINT, PPARBP, and MeCP2. See also Figure S6.

Journal: Chemistry & biology

Article Title: Specificity analysis-based identification of new methylation targets of the SET7/9 protein lysine methyltransferase.

doi: 10.1016/j.chembiol.2010.11.014

Figure Lengend Snippet: Figure 6. SET7/9 Is Inhibited by Phosphorylation of the Target Peptide or Protein (A) Relative activity of SET7/9 on CelluSpot array derived from H3 peptides containing different combinations of PTMs (Table S2). Phosphorylation of T3, S10, and T11 abolished SET7/9 activity. (B) Influence of phosphorylation of nonhistone substrates on SET7/9 methyla- tion activity. GST-tagged nonhistone substrates of SET7/9 were incubated with PKA. All domains except Cullin1 were phosphorylated (Figure S5). Then, phosphorylated and mock treated domains were methylated by SET7/9. A complete loss or strong reduction of SET7/9 activity was observed with ZDN8, IRF1, MINT, PPARBP, and MeCP2. See also Figure S6.

Article Snippet: Cloning, Expression, and Purification of Proteins The sequence encoding human A kinase anchor protein 6 (AKA6) (residues 485–776; NCBI accession number NP_004265.3), Bromodomain containing 1 (BRD1) (residues 681–891; NCBI accession number NP_055392.1), Centromeric protein C1 (CENPC1) (residues 279–474; NCBI accession number NP_001803.2), Dnmt1 (residues 622–780; NCBI accession number NP_001370.1), Methyl CpG binding protein 2 (MeCP2) (residues 201–486; NCBI accession number NP_004983.1), Msx2-interacting protein (MINT) (residues 1994–2281; NCBI accession number NP_055816.2), PPAR binding protein (PPARBP) (residues 847–1084; NCBI accession number NP_004765.2), Retinoblastoma binding protein 5 (RBBP5) (residues 323– 538; NCBI accession number NP_005048.2), Zinc finger DHHC domain containing 8 (ZDHHC8) (residues 227–400; NCBI accession number NP_037505.1), PRP4 pre-mRNA processing factor 4 homolog B (PRP4) (residues 93–396; NCBI accession number NP_789770.1) CDC2 related protein kinase 7 (CRKRS) (residues 2–339; NCBI accession number NP_057591.1), Cullin1 (residues 2–250; NCBI accession number NP_003583.2), TTK (residues 603–799; NCBI accession number NP_003309.2), and IRF1 (residues 71–240; 118 Chemistry & Biology 18, 111–120, January 28, 2011 a2011 Elsev NCBI accession number NP_002189.1) were amplified from HEK293 cDNA and cloned into pENTR/D-TOPO (Invitrogen) gateway entry vector according to the manufacturer’s instructions. pGEX-6P-2 vector (GE healthcare) was converted into Gateway destination vector (pGEX-6P-2-GW-AJ) using Gateway vector conversion system (Invitrogen).

Techniques: Phospho-proteomics, Activity Assay, Derivative Assay, Incubation, Methylation